Cambridge, United Kingdom (29 October 2010) – One new Blooms Syndrome Protein (BLM) antibody made to a phosphorylated site was recently introduced to the bioscience research industry by Novus Biologicals. The Blm [phospho Thr99] antibody (catalog # NBP1-46851) recognizes the Bloom Syndrome Protein at threonine 99. This new pBLM antibody has been twice published and is backed by Novus’ Guarantee + Innovator’s Reward™ Policy.
Novus’ new phospho-BLM [Thr99] antibody cross-reacts with human and is useful for Western blot analysis, where it recognizes a band at ~160kDa. Western blot analysis was conducted in MDA-MB-231 cells, including non-treated cells and cells treated with 1uM camptothecin.
The Bloom’s syndrome gene product, BLM protein, is a member of the RecQ helicase protein family. BLM protein contains 1417 amino acids; including one ATP binding site, one DEAH box, and two putative nuclear localization signals, as well as phosphorylation sites. BLM protein is known to associate, and be phosphorylated by the ATR protein in response to genetic stress. The BLM protein is located in the nucleus of normal human cells in the nuclear domain 10 (ND10) or promyelocytic leukemia nuclear (PML) bodies. These structures are punctate deposits of proteins disrupted upon viral infection and in certain human malignancies. The BLM protein is likely to be part of a DNA surveillance mechanism operating during S phase – BLM was found to be part of the BASC (BRCA1-associated genome surveillance) complex, which may serve as a sensor of abnormal DNA structures and/or as a regulator of the postreplication repair process. The BLM protein is thought to play a role in the etiology of cancer and diabetes, two major human health problems that are common complications of Bloom’s syndrome.
Novus provides other Blooms Syndrome Protein antibodies, proteins and RNAi which can be found here. BLM -related products are also available from Novus, including: c-Kit antibodies, Rad51 antibodies, TNF alpha antibodies, and WRN antibodies.
For more information on this phospho-BLM antibody or to inquire about product collaborations, please contact the Novus Product Development Team by calling 303-730-1950 or via e-mail at collaborations@novusbio.com.
For more details about Novus Biologicals, please visit their website at http://www.novusbio.com, or call +44 (0)1223 426001 (Europe) or 1-888-50-NOVUS (USA).
About Novus Biologicals:
Novus Biologicals is an international scientific company, helping to accelerate the advancement of scientific discoveries by developing and commercialising new products in scientific and biological fields. By promoting and commercialising unique biological materials and products to the biological research community, Novus plays an important part in helping to advance international biological research practices. Novus is also constantly monitoring emerging scientific trends and strives to supply products for their advancement.
Contact Details:
Amy Chamberlain-Torres, MBA
Product Marketing Manager
Novus Biologicals Europe
12 Cambridge Science Park
Cambridge, CB4 0FQ, UK
Tel: +44 (0)1223 426001
Fax: +44 (0)871 971 1635
Email: europe@novusbio.com
Novus Biologicals Worldwide
PO Box 802
Littleton, CO 80160 USA
Tel: 1-888-50-NOVUS
Fax: 303-730-1966
Email: novus@novusbio.com
